PHOTOSYNTHESIS  role play Splitting water Electron transport Chain ATP formation Calvin cycle

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THINGS YOU WILL NEED:
Roll of Velcro with adhesive on back
yarn
hole puncher
Flashlight

AHEAD OF TIME
PHOTOSYNTHESIS THEATER CARDS BY AUDRA WARD BROWN
1. Copy off the role play cards onto cardstock or colored paper and laminate them.
2. Cut apart the e^-, P, H⁺ , and CO₂ cards.

3. Use a paper punch to put holes in the following cards and attach string so card can be worn around the neck. (Photosystem I & II, ATP synthase, Stroma, Thylakoid space, ETC,  NADP+, Adenosine P P , O, Calvin cycle)

4. Cut small strips of Velcro at attach the hook side to the front of to Adenosine P P card and the loop side to the back of the P circle so it can be attached to make ATP. Cut the "hook" strip about 1/2 the size of the "loop" side. Otherwise they grab too much and rip off easily.

5. Do the same with the NADP⁺  and the H⁺ cards.

6. Do the same with the O card to make places to attach 2 H+ to make a H2O molecule.

MINIMUM # OF CARDS YOU WILL NEED:

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ACT IT OUT: LIGHT DEPENDENT REACTIONS

Students wear appropriate name tags and line up in correct position.
Photosystem II, ETC, ETC, ETC, Photosystem I, ETC, ATP synthase stand in a line
Sun stands to side of PS II.
2 Stroma people stand in front of line holding baskets
2 Thylakoid space people stand behind line holding baskets
Water stands next to thylakoid space people.
ADP stands near ATP synthase
NADP⁺ stands near ATP synthase and last ETC

Roles: 
Sun: Holds flashlight and shines it on Photosystems
PS II: passes e^- cards to first ETC
ETC: Passes e^- cards along line and pumps H⁺ cards over shoulder to thylakoid space
PS I: Passes e^- cards to LAST ETC
WATER: Each starts wearing an O card with 2 H's attached and holds 2 e- cards.
     Removes H⁺ from O name card & gives to thylakoid space; Passes e- cards to PS II; 2 O's join arms and leave room
STROMA: Passes H⁺  cards to ETC; Receive H cards from ATP SYNTHASE; Pass H⁺  cards to last ETC
THYLAKOID SPACE: Receives H⁺ cards from ETC and passes H⁺ cards to ATP synthase
ATP SYNTHASE:  receives H⁺ cards from thylakoid space and passes them to stroma; holds P cards and attaches them to ADP.
LAST ETC: collects all e^- cards as they come down line, receives H⁺ from stroma and gives 2 e^- cards and attaches an H⁺ to NADP⁺

ACT OUT ETC first. Where is this happening? What are the jobs? Where do electrons come from to replace those lost from PS II? Where are ATP and NADPH made? Where does H⁺ build up? Where do ATP and NADPH go next?

Act it out, then have students change jobs.

Fill out What's my job when done.

Then add in SPLITTING WATER and CALVIN CYCLE.

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SPLITTING WATER:
Assign 8 students to be water molecules in THYLAKOID SPACE.

As water is split, the H⁺ cards are detached and are passed to the THYLAKOID SPACE.
The 2 e- cards pass to PS II, and the leftover O holds hands and joins with another O to become O₂ and leaves the chloroplast (room).

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CALVIN CYCLE:What happens to the NADPH and ATP made by light reactions?

Actors:
12 students each hold 1 CO₂ card, ATP and NADPH from light reactions

CO₂, ATP and NADPH travel to CALVIN CYCLE.
ATP removes its P, NADPH removes its H⁺ and passes it and 2 e^- cards to CALVIN CYCLE. 
CO₂'s hand their cards to CALVIN CYCLE.

CALVIN CYCLE takes 2 e^- and H⁺ from NADPH, and energy and P from ATP and
CO₂'s  hold  their cards and CALVIN CYCLE links their arms to form a 6 person ring*
   OR they can all form a ring and hold their CO2 cards together in the middle.

* If you want to get technical . . . it should be a 5 person ring with one "flag" C

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